Exosome Biotinylation

In order to better understand exosome features and exosome trafficking for therapeutic and diagnostic purposes, exosome biotin labeling is a useful tool.

A protein, nucleic acid, or other molecule can bind to biotin, a tiny molecule, covalently. This procedure, known as biotinylation, is quick, precise, and unlikely to affect the way that proteins and other target molecules normally work. Streptavidin and avidin are non-covalently bound by biotin with extraordinarily high affinity, a rapid on-rate, and excellent specificity. Additionally, non-covalent contacts are extremely potent—even stronger than the affinity between antibodies and antigens—and resistant to high temperatures, acidic pH levels, and proteolysis.